|
Field of View |
Horizontal Intensity Profile |
Vertical Intensity Profile |
10X: Exposure time 700 μsec. |
|
 |
20X: Exposure time 400 μsec. |
 |
 |
Home > LED Light Sources > Uniformity of Illumination with 030-457 Light Source
Uniformity of Illumination with TOFRA 030-457 LED Light Source
Tests were performed on Olympus BX51 microscope with 10X UPlanFL 0.3NA and 20X UPlanFL 0.5NA objectives and Chroma 39002 - AT - EGFP/FITC/Cy2/AlexaFluor 488 filter cube.
Camera - Basler color camera scA 1400-17gc, chip size 1390*1038 pixels, pixel size 6.45 μm * 6.45 μm, in 8-bit mode. Gain 200.
Intensity profiles were drawn through the center of the frame and
averaged over 20 rows (columns).
Specimen: Chroma Green Autofluorescent Plastic Slide.
|
Field of View |
Horizontal Intensity Profile |
Vertical Intensity Profile |
10X: Exposure time 700 μsec. |
|
|
20X: Exposure time 400 μsec. |
|
|
